Vértessy G. Beáta MTA bemutatkozás


Kiemelt 4 közlemény (az adott közlemény a címre kattintva nyílik meg)

 1. Vértessy BG. Flexible glycine rich motif of Escherichia coli deoxyuridine triphosphate nucleotidohydrolase is important for functional but not for structural integrity of the enzyme. Proteins. 1997 Aug;28(4):568-79.

2. Barabás O, Pongrácz V, Kovári J, Wilmanns M, Vértessy BG. Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase. J Biol Chem. 2004 Oct 8;279(41):42907-15.

3. Muha V, Horváth A, Békési A, Pukáncsik M, Hodoscsek B, Merényi G, Róna G, Batki J, Kiss I, Jankovics F, Vilmos P, Erdélyi M, Vértessy BG. Uracil-containing DNA in Drosophila: stability, stage-specific accumulation, and developmental involvement. PLoS Genet. 2012;8(6):e1002738.

4. Róna G, Marfori M, Borsos M, Scheer I, Takács E, Tóth J, Babos F, Magyar A, Erdei A, Bozóky Z, Buday L, Kobe B, Vértessy BG. Phosphorylation adjacent to the nuclear localization signal of human dUTPase abolishes nuclear import: structural and mechanistic insights. Acta Crystallogr D Biol Crystallogr. 2013 Dec; 69 (Pt 12):2495-505.



Kiegészítő dokumentáció MTA levelező tagjelölti ajánláshoz

Vértessy G. Beáta

 

 

Hazai, önállóan végzett kutatásaimban vagy hazai saját kutatócsoportom keretében, korábbi mentoromtól/témavezetőmtől függetlenül született olyan cikkeim száma, amelyekben első vagy utolsó és/vagy levelező szerző vagyok:



74 cikk, ebből 69-ben eredeti in extenso, kísérletes munkákból született eredményeket közöltem, összesen 4 review és 1 “response” cikk van köztük.


Ebből kiemelt presztízsű D1-es folyóiratban megjelent publikáció: 31 (ezeket alább vastag betűvel emeltem ki)


A cikkeket ebben a dokumentumban fel is sorolom. Ezek a cikkek korábbi témavezetőimtől függetlenül születtek, amit a szerzőlista áttekintésével és korábbi témavezetőim (ld alábbi felsorolás) ismeretében lehet igazolni.


Szakmai pályafutásom során témavezetőim, mentoraim voltak a következő kutatók:

Dr Keleti Tamás (MTA Enzimológiai Intézet) – MTA TMB ösztöndíjas időszakom alatti témavezetőm, MTA kandidátusi disszertációm irányítója 1984-1989 között

Kazinczyné Dr Vas Mária (MTA Enzimológiai Intézet) – témavezetőm egyetemi éveim alatt végzett TDK munkám során (1982-1984), továbbá rész-témavezetőm MTA TMB ösztöndíjas időszakom alatt (1984-1986)

Dr Theodore Steck (University of Chicago) – témavezetőm a Univ Chicagon töltött 1986-87 tanév során

Dr Ovádi Judit (MTA Enzimológiai Intézet– rész-témavezetőm MTA TMB ösztöndíjas időszakom alatt (1987-1989), továbbá témavezetőm hazai posztdoktori éveim alatt 1991-1992, 1994-1998 között

Dr Michael Zeppezauer (Universitat Saarlandes) – témavezetőm 1992-1994 között (Alexander von Humboldt posztdoktori időszakom alatt)



Cikkek listája


74: Nyiri K, Mertens HDT, Tihanyi B, Nagy GN, Kohegyi B, Matejka J, Harris MJ, Szabo JE, Papp-Kadar V, Nemeth-Pongracz V, Ozohanics O, Vekey K, Svergun DI, Borysik AJ, Vertessy BG

Structural model of human dUTPase in complex with a novel proteinaceous inhibitor

SCIENTIFIC REPORTS 8:(1) Paper 4326. 15 p. (2018)

 

73: Marton L, Hajdu F, Nagy GN, Kucsma N, Szakacs G, Vertessy BG

Heterologous expression of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum rescues Chinese Hamster Ovary cells deficient in the Kennedy phosphatidylcholine biosynthesis pathway

SCIENTIFIC REPORTS 8: Paper 8932. 8 p. (2018)

 

 

72: Molnár P, Marton L, Izrael R, Pálinkás HL, Vértessy BG

Uracil moieties in Plasmodium falciparum genomic DNA

FEBS OPEN BIO First Published:27 May 2018 https://doi.org/10.1002/2211-5463.1245872:

 

71: Andras Benedek, Istvan Poloskei, Oliver Ozohanics, Karoly Vekey, Beata G Vertessy

The Stl repressor from Staphylococcus aureus is an efficient inhibitor of the eukaryotic fruitfly dUTPase

FEBS OPEN BIO 8:(2) p. 158167. 10 p. (2018) (címlapábra)

 

70: Surányi EV*, Hírmondó R*, Nyíri K, Tarjányi S, Kőhegyi B, Tóth J, Vertessy BG* (* corr authors)

Exploiting a phage-bacterium interaction system as a molecular switch to decipher macromolecular interactions in the living cell

VIRUSES 10:(4) Paper 168. 13 p. (2018)

 

69: Zsófia Bata, Renzhe Qian, Alexander Roller, Jeannie Horak, László Csaba Bencze, Csaba Paizs, Friedrich Hammerschmidt, Beáta G Vértessy*, László Poppe* (* corr author)

A Methylidene Group in Phosphonic Acid Analogue of Phenylalanine Reverses the Enantiopreference of Binding to Phenylalanine Ammonia-Lyases

ADVANCED SYNTHESIS & CATALYSIS (2017) 359(12):2109-2120.

 

68: Kenesi E, Carbonell A, Lózsa R, Vértessy B*, Lakatos L*. (*corr. authors)

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC.

Nucleic Acids Res. 2017 May 12. doi: 10.1093/nar/gkx379. [Epub ahead of print] PubMed PMID: 28499009.

 

REVIEW CIKK: 67: Nyíri K, Vértessy BG.

Perturbation of genome integrity to fight pathogenic microorganisms.

Biochim Biophys Acta. 2017 Jan;1861(1 Pt B):3593-3612.

 

65: Kerepesi C, Szabó JE, Papp-Kádár V, Dobay O, Szabó D, Grolmusz V, Vértessy BG.

Life without dUTPase.

Front Microbiol. 2016 Nov 14;7:1768. 

 

64: Lopata A, Leveles I, Bendes ÁÁ, Viskolcz B, Vértessy BG*, Jójárt B*, Tóth J*. (* corresponding authors)

A Hidden Active Site in the Potential Drug Target Mycobacterium tuberculosis dUTPase Is Accessible through Small Amplitude Protein Conformational Changes.

J Biol Chem. 2016 Dec 16;291(51):26320-26331. 

 

63: Nagy GN, Suardíaz R, Lopata A, Ozohanics O, Vékey K, Brooks BR, Leveles I, Tóth J, Vértessy BG­*, Rosta E*. (* corresponding authors)

Structural Characterization of Arginine Fingers:

Identification of an Arginine Finger for the Pyrophosphatase dUTPases.

J Am Chem Soc. 2016 Nov 16;138(45):15035-15045.

 

62: Papp-Kádár V, Szabó JE, Nyíri K, Vertessy BG.

In Vitro Analysis of Predicted DNA-Binding Sites for the Stl Repressor of the Staphylococcus aureus SaPIBov1 Pathogenicity Island.

PLoS One. 2016 Jul 7;11(7):e0158793.

 

61: Benedek A, Horváth A, Hirmondó R, Ozohanics O, Békési A, Módos K, Révész Á, Vékey K, Nagy GN, Vértessy BG.

Potential steps in the evolution of a fused trimeric all-β dUTPase involve a catalytically competent fused dimeric intermediate.

FEBS J. 2016 Sep;283(18):3268-86. “Editor’s Choice”

 

60: Pukáncsik M, Orbán Á, Nagy K, Matsuo K, Gekko K, Maurin D, Hart D, Kézsmárki I, Vertessy BG.

Secondary Structure Prediction of Protein Constructs Using Random Incremental Truncation and Vacuum-Ultraviolet CD Spectroscopy.

PLoS One. 2016 Jun 7;11(6):e0156238.


59: Róna G, Scheer I, Nagy K, Pálinkás HL, Tihanyi G, Borsos M, Békési A, Vértessy BG.

Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications.

Nucleic Acids Res. 2016 Feb 18;44(3):e28.

 

58: Nyíri K, Kőhegyi B, Micsonai A, Kardos J, Vertessy BG.

Evidence-Based Structural Model of the Staphylococcal Repressor Protein: Separation of Functions into Different Domains.

PLoS One. 2015 Sep 28;10(9):e0139086.

 

57: Nyíri K, Papp-Kádár V, Szabó JE, Németh V, Vértessy BG

Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase

Structural Chemistry 2015. 26 (5-6): 1425-1432

 

56: Marton L, Nagy GN, Ozohanics O, Lábas A, Krámos B, Oláh J, Vékey K, Vértessy BG.

Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:phosphocholine Cytidylyltransferase

Plos One, 2015 Jun 17;10(6):e0129632.

 

55: Hirmondó R, Szabó JE, Nyíri K, Tarjányi S, Dobrotka P, Tóth J, Vértessy BG.

Cross-species inhibition of dUTPase via the Staphylococcal Stl protein perturbs dNTP pool and colony formation in Mycobacterium.

DNA Repair (Amst). 2015 Mar 19;30:21-27.

 

54: Horváth A, Batki J, Henn L, Lukacsovich T, Róna G, Erdélyi M, Vértessy BG.

dUTPase expression correlates with cell division potential in Drosophila melanogaster.

FEBS J. 2015 Mar 4. doi: 10.1111/febs.13255.

 

53. Lopata A, Jambrina PG, Sharma PK, BR Brooks BR, Toth J, Vertessy BG*, Rosta E*. (* corresponding authors)

Mutations decouple proton transfer from phosphate cleavage in the dUTPase catalytic reaction

ACS Catalysis 2015 Apr 8;5:3225-3237 


52: Róna G, Borsos M, Ellis JJ, Mehdi AM, Christie M, Környei Z, Neubrandt M, Tóth J, Bozóky Z, Buday L, Madarász E, Bodén M, Kobe B, Vértessy BG.

Dynamics of re-constitution of the human nuclear proteome after cell division is regulated by NLS-adjacent phosphorylation.

Cell Cycle. 2014;13(22):3551-64.

 

RESPONSE CIKK”: 51: Róna G, Borsos M, Kobe B, Vértessy BG.

Factors influencing nucleo-cytoplasmic trafficking: which matter?

Acta Crystallogr D Biol Crystallogr.2014 Oct;70(Pt 10):2777-8.

 

 

50: Nagy GN, Marton L, Contet A, Ozohanics O, Ardelean LM, Révész A, Vékey K, Irimie FD, Vial H, Cerdan R, Vértessy BG.

Composite aromatic boxes for enzymatic transformations of quaternary ammonium substrates.

Angew Chem Int Ed Engl. 2014 Dec 1;53(49):13471-6

 

 

49: Róna G, Pálinkás HL, Borsos M, Horváth A, Scheer I, Benedek A, Nagy GN, Zagyva I, Vértessy BG.

NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases.

FEBS J. 2014 Dec;281(24):5463-78. 

 

48: Szabó JE, Németh V, Papp-Kádár V, Nyíri K, Leveles I, Bendes AÁ, Zagyva I, Róna G, Pálinkás HL, Besztercei B, Ozohanics O, Vékey K, Liliom K, Tóth J, Vértessy BG.

Highly potent dUTPase inhibition by a bacterial repressor protein reveals a novel mechanism for gene expression control.

Nucleic Acids Res. 2014 Oct 29;42(19):11912-20.

 

 

47: Szalkai B, Scheer I, Nagy K, Vértessy BG*, Grolmusz V*.

(* corresponding authors)

The metagenomic telescope.

PLoS One. 2014 Jul 23;9(7):e101605.


REVIEW CIKK: 46: Nagy GN, Leveles I, Vértessy BG.

Preventive DNA repair by sanitizing the cellular (deoxy)nucleoside triphosphate pool.

FEBS J. 2014 Sep;281(18):4207-23.

  

45: Kovács K, Bánóczi G, Varga A, Szabó I, Holczinger A, Hornyánszky G, Zagyva I, Paizs C, Vértessy BG*, Poppe L*. (* corresponding authors)

Expression and properties of the highly alkalophilic phenylalanine ammonia-lyase of thermophilic Rubrobacter xylanophilus.

PLoS One. 2014 Jan 27;9(1):e85943.


44: Magazu S, Migliardo F, Benedetto A, Vertessy B

Protein dynamics by neutron scattering: The protein dynamical transition and the fragile-to-strong dynamical crossover in hydrated lysozyme

CHEMICAL PHYSICS 424: pp. 26-31. (2013)

 

43: Róna G, Marfori M, Borsos M, Scheer I, Takács E, Tóth J, Babos F, Magyar A, Erdei A, Bozóky Z, Buday L, Kobe B, Vértessy BG.

Phosphorylation adjacent to the nuclear localization signal of human dUTPase abolishes nuclear import: structural and mechanistic insights.

Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt12):2495-505. 

 

42: Leveles I, Németh V, Szabó JE, Harmat V, Nyíri K, Bendes ÁÁ, Papp-Kádár V, Zagyva I, Róna G, Ozohanics O, Vékey K, Tóth J, Vértessy BG.

Structure and enzymatic mechanism of a moonlighting dUTPase.

Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2298-308.

 

 

41: Barabás O, Németh V, Bodor A, Perczel A, Rosta E, Kele Z, Zagyva I, Szabadka Z, Grolmusz VI, Wilmanns M, Vértessy BG.

Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling.

Nucleic Acids Res. 2013 Dec;41(22):10542-55.

 

40: Nagy GN, Marton L, Krámos B, Oláh J, Révész Á, Vékey K, Delsuc F, Hunyadi-Gulyás É, Medzihradszky KF, Lavigne M, Vial H, Cerdan R, Vértessy BG.

Evolutionary and mechanistic insights into substrate and product accommodation of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum.

FEBS J. 2013 Jul;280(13):3132-48.

 

39: Horváth A, Békési A, Muha V, Erdélyi M, Vértessy BG.

Expanding the DNA alphabet in the fruit fly: uracil enrichment in genomic DNA.

Fly (Austin). 2013 Jan-Mar;7(1):23-7.

 

38: Muha V, Horváth A, Békési A, Pukáncsik M, Hodoscsek B, Merényi G, Róna G, Batki J, Kiss I, Jankovics F, Vilmos P, Erdélyi M, Vértessy BG.

Uracil-containing DNA in Drosophila: stability, stage-specific accumulation, and developmental involvement.

PLoS Genet. 2012;8(6):e1002738.

 

37: Pecsi I, Hirmondo R, Brown AC, Lopata A, Parish T, Vertessy BG*, Tóth J*. (* corresponding authors)

The dUTPase enzyme is essential in Mycobacterium smegmatis.

PLoS One. 2012;7(5):e37461.

 

36: Magazù S, Migliardo F, Gonzalez MA, Mondelli C, Parker SF, Vertessy BG.

Molecular mechanisms of survival strategies in extreme conditions.

Life (Basel). 2012 Dec 7;2(4):364-76.

 

35: Poppe L, Paizs C, Kovács K, Irimie FD, Vértessy B.

Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases.

Methods Mol Biol.2012;794:3-19. 

 

34: Leveles I, Róna G, Zagyva I, Bendes Á, Harmat V, Vértessy BG.

Crystallization and preliminary crystallographic analysis of dUTPase from the φ11 helper phage of Staphylococcus aureus.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1411-3.

 

33: Pécsi I, Szabó JE, Adams SD, Simon I, Sellers JR, Vértessy BG*, Tóth J*. (* corresponding authors)

Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination.

Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14437-42.

 

32: Merényi G, Kovári J, Tóth J, Takács E, Zagyva I, Erdei A, Vértessy BG.

Cellular response to efficient dUTPase RNAi silencing in stable HeLa cell lines perturbs expression levels of genes involved in thymidylate metabolism.

Nucleosides Nucleotides Nucleic Acids. 2011 Jun;30(6):369-90.

 

 

31: Bozóky Z, Róna G, Klement É, Medzihradszky KF, Merényi G, Vértessy BG*, Friedrich P*. (* corresponding authors)

Calpain-catalyzed proteolysis of human dUTPase specifically removes

the nuclear localization signal peptide.

PLoS One. 2011;6(5):e19546. 

 

30: Erdélyi P, Borsos E, Takács-Vellai K, Kovács T, Kovács AL, Sigmond T, Hargitai B, Pásztor L, Sengupta T, Dengg M, Pécsi I, Tóth J, Nilsen H, Vértessy BG*, Vellai T*. (* corresponding authors)

Shared developmental roles and transcriptional control of autophagy and apoptosis in Caenorhabditis elegans.

J Cell Sci. 2011 May 1;124(Pt 9):1510-8. 

 

29: Bekesi A, Pukancsik M, Haasz P, Felfoldi L, Leveles I, Muha V, Hunyadi-Gulyas E, Erdei A, Medzihradszky KF, Vertessy BG.

Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding.

FEBS J. 2011 Jan;278(2):295-315.

 

REVIEW CIKK: 28: Vértessy BG, Orosz F.

From "fluctuation fit" to "conformational selection": evolution, rediscovery, and integration of a concept.

Bioessays. 2011 Jan;33(1):30-4. 


27: Horváth A, Vértessy BG.

A one-step method for quantitative determination of uracil in DNA by real-time PCR.

Nucleic Acids Res. 2010 Nov;38(21):e196.

 

 

26: Pecsi I, Leveles I, Harmat V, Vertessy BG*, Toth J*. (*corresponding authors)

Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase.

Nucleic Acids Res. 2010 Nov;38(20):7179-86.

 

25: Takács E, Nagy G, Leveles I, Harmat V, Lopata A, Tóth J, Vértessy BG. Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases.

FEBS Lett. 2010 Jul 16;584(14):3047-54. 

 

24: Merényi G, Kónya E, Vértessy BG.

Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals.

FEBS J.2010 May;277(9):2142-56. 

 

23: Pukáncsik M, Békési A, Klement E, Hunyadi-Gulyás E, Medzihradszky KF,

Kosinski J, Bujnicki JM, Alfonso C, Rivas G, Vértessy BG.

Physiological truncation and domain organization of a novel uracil-DNA-degrading factor.

FEBS J. 2010 Mar;277(5):1245-59. 

 

22: Takács E, Barabás O, Petoukhov MV, Svergun DI, Vértessy BG. Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers.

FEBS Lett. 2009 Mar 4;583(5):865-71. 

 

21: Muha V, Zagyva I, Venkei Z, Szabad J, Vértessy BG.

Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage.

Biochem Biophys Res Commun. 2009 Apr 3;381(2):271-5.

 

REVIEW CIKK: 20: Vértessy BG, Tóth J.

Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases.

Acc Chem Res. 2009 Jan 20;42(1):97-106. 

 

19: Varga B, Barabás O, Takács E, Nagy N, Nagy P, Vértessy BG.

Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor.

Biochem Biophys Res Commun. 2008 Aug 15;373(1):8-13. 

 

18: Kovári J, Barabás O, Varga B, Békési A, Tölgyesi F, Fidy J, Nagy J, Vértessy BG.

Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site.

Proteins. 2008 Apr;71(1):308-19. PubMed PMID: 17932923.

 

 

17: Varga B, Barabás O, Kovári J, Tóth J, Hunyadi-Gulyás E, Klement E, Medzihradszky KF, Tölgyesi F, Fidy J, Vértessy BG.

Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase.

FEBS Lett. 2007 Oct 2;581(24):4783-8. Epub 2007 Sep 12.

 

16: Tóth J, Varga B, Kovács M, Málnási-Csizmadia A, Vértessy BG.

Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme.

J Biol Chem. 2007 Nov 16;282(46):33572-82. 

 

15: Békési A, Pukáncsik M, Muha V, Zagyva I, Leveles I, Hunyadi-Gulyás E, Klement E, Medzihradszky KF, Kele Z, Erdei A, Felföldi F, Kónya E, Vértessy BG.

A novel fruitfly protein under developmental control degrades uracil-DNA.

Biochem Biophys Res Commun. 2007 Apr 13;355(3):643-8. 

 

14: Németh-Pongrácz V, Barabás O, Fuxreiter M, Simon I, Pichová I, Rumlová M, Zábranská H, Svergun D, Petoukhov M, Harmat V, Klement E, Hunyadi-Gulyás E, Medzihradszky KF, Kónya E, Vértessy BG.

Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins.

Nucleic Acids Res. 2007;35(2):495-505.

 

13: Németh-Pongrácz V, Snasel J, Rumlova M, Pichova I, Vértessy BG.

Interacting partners of M-PMV nucleocapsid-dUTPase.

Nucleosides Nucleotides Nucleic Acids.

2006;25(9-11):1197-200. PubMed PMID: 17065090.

 

12: Barabás O, Németh V, Vértessy BG.

Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt 4):399-401.

 

11: Kovári J, Imre T, Szabó P, Vértessy BG.

Mechanistic studies of dUTPases.

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(8-9):1475-9. 

 

10: Barabás O, Pongrácz V, Kovári J, Wilmanns M, Vértessy BG.

Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase.

J Biol Chem. 2004 Oct 8;279(41):42907-15. “Paper of the week”

 

9: Takács E, Grolmusz VK, Vértessy BG.

A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases.

FEBS Lett. 2004 May21;566(1-3):48-54. PubMed PMID: 15147867.

 

 

8: Békési A, Zagyva I, Hunyadi-Gulyás E, Pongrácz V, Kovári J, Nagy AO, Erdei A,Medzihradszky KF, Vértessy BG.

Developmental regulation of dUTPase in Drosophila melanogaster.

J Biol Chem. 2004 May 21;279(21):22362-70. Epub 2004 Mar 2. PubMed  

 

7: Kovári J, Barabás O, Takács E, Békési A, Dubrovay Z, Pongrácz V, Zagyva I,Imre T, Szabó P, Vértessy BG.

Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and

crystallographic studies of the homotrimeric Drosophila enzyme.

J Biol Chem. 2004 Apr 23;279(17):17932-44. Epub 2004 Jan 14. PubMed PMID: 14724274.

 

6: Dubrovay Z, Gáspári Z, Hunyadi-Gulyás E, Medzihradszky KF, Perczel A,

Vértessy BG.

Multidimensional NMR identifies the conformational shift essential for catalytic competence in the 60-kDa Drosophila melanogaster dUTPase trimer.

J Biol Chem. 2004 Apr 23;279(17):17945-50. Epub 2004 Jan 14. PubMed PMID: 14724273.

  

5: Barabás O, Rumlová M, Erdei A, Pongrácz V, Pichová I, Vértessy BG. dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency.

J Biol Chem. 2003 Oct 3;278(40):38803-12. Epub 2003 Jul 16. PubMed PMID: 12869552.

 

4: Mustafi D, Bekesi A, Vertessy BG*, Makinen MW*. (* corresponding authors)

Catalytic and structural role of the metal ion in dUTP pyrophosphatase.

Proc Natl Acad Sci U S A. 2003 May 13;100(10):5670-5. Epub 2003 Apr 29. PubMed PMID: 12721364; PubMed Central PMCID: PMC156259.

 

3: Fiser A, Vértessy BG.

Altered subunit communication in subfamilies of trimeric dUTPases.

Biochem Biophys Res Commun. 2000 Dec 20;279(2):534-42. PubMed

PMID: 11118321.

 

2: Vertessy BG*, Larsson G, Persson T, Bergman AC, Persson R, Nyman PO. The complete triphosphate moiety of non-hydrolyzable substrate analogues is required for a conformational shift of the flexible C-terminus in E. coli dUTP pyrophosphatase. (* corresponding author)

FEBS Lett. 1998 Jan 2;421(1):83-8. PubMed PMID: 9462846. - címlapábra

 

1: Vertessy BG.

Flexible glycine rich motif of Escherichia coli deoxyuridine triphosphate nucleotidohydrolase is important for functional but not for structural integrity of the enzyme. Proteins. 1997 Aug;28(4):568-79. PubMed PMID: 9261872.



1